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Journal: The Journal of Experimental Medicine
Article Title: Anti-human TREM2 induces microglia proliferation and reduces pathology in an Alzheimer’s disease model
doi: 10.1084/jem.20200785
Figure Lengend Snippet: Impact of AL002c treatment on total Aβ load. (A) Quantification of methoxy-X04 + area coverage in the cortex (Ctx) and hippocampus (HC) of treated mice. Each CV-KO-5XFAD treatment group, n = 7; each R47H-KO-5XFAD treatment group, n = 6. (B) Volume of individual methoxy-X04 + Aβ plaques in the cortex of indicated treatment groups. The volume of ∼1,000 plaques per condition was determined by Imaris. (C–F) PBS-soluble and PBS-insoluble guanidine-soluble fractions of hippocampus were assessed for Aβ 1–40 (C and E) and Aβ 1–42 (D and F) by MSD. Values for PBS-soluble Aβ 1–40 were below the limit of detection in some samples. Each CV-KO-5XFAD treatment group, n = 7; each R47H-KO-5XFAD treatment group, n = 6. No differences were detected for Aβ 1–40 or Aβ 1–42 in PBS-insoluble guanidine-soluble fractions of cortices among treatment groups (not depicted). PBS-soluble fractions of cortices showed no detectable Aβ 1–40 or Aβ 1–42 by MSD . *, P < 0.05; **, P < 0.01 by two-way ANOVA. Data are presented as mean ± SEM.
Article Snippet: Aβ 1–40 and Aβ 1–42 levels were measured in both fractions by the
Techniques: